Phosphinothricin acetyltransferase

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August undefined, 2024

WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... WebDonn G, Dirks R, Eckes P, Uijtewaal B (1990a) Transfer and expression of modified phosphinothricin-acetyltransferase gene from Streptomyces viridichromogenes in tomato, melon, carrot, and strawberry. Abstr VII Int Congr Plant Tissue Cell Cult Amsterdam: 176.

Use of bar as a selectable marker gene and for the production of ...

WebReconstitution. It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH 2 O. Storage & Stability. Stored at -20°C. After reconstitution, it is stable at … WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of … solihull borough council planning

Safety evaluation of the phosphinothricin acetyltransferase …

WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate … WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from … Webl -Phosphinothricin ( l -Pt)-resistant plants were constructed by introducing a modified phosphinothricin- N -acetyl-transferase gene ( pat) via Agrobacterium -mediated gene … solihull borough

phosphinothricin acetyltransferase (PAT) ELISA Kits

Phosphinothricin acetyltransferase - Wikiwand

WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) fluthiacet-methyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions … WebMay 26, 2024 · Phosphinothricin acetyltransferase (PAT), the target protein (Kim et al. 2010) investigated in this study, is a typical herbicide-resistant protein coded by a bar gene isolated from Streptomyces hygroscopicus … solihull bowlingWebFilters carrying the bombarded tissues were selected on basal medium with increasing concentrations (maximum of 200 mg I-1) of phosphinothricin. Surviving calli were assayed for the presence of phosphinothricin acetyltransferase activity. All positive calli were shown to have the bar gene integrated into their genome. Nine independent stable ... small balance charge off

"WebOct 1, 1996 · The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant … " - Phosphinothricin acetyltransferase

Phosphinothricin acetyltransferase

Transgenic sorghum plants via microprojectile bombardment.

WebPhosphinothricin acetyltransferase is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase.[1][2] This enzyme catalyses the following … WebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower …

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WebDec 1, 1993 · Phosphinothricin acetyltransferase activity was detected in extracts of the T0 plants. These plants were resistant to local application of the herbicide Ignite/Basta, and the resistance was inherited in T1 plants as a single dominant locus. WebProduct: phosphinothricin acetyltransferase. confers resistance to bialophos or phosphinothricin: BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa. Product: phosphinothricin acetyltransferase. confers resistance to bialophos or …

WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase (PAT) and bialaphos resistance (bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology, generation of … WebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for …

Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector …

WebPPT N-acetyltransferase. acetyl-CoA + phosphinothricin <=> CoA + H (+) + N-acetylphosphinothricin. The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, a key enzyme of nitrogen metabolism in plants. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in ...

WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... solihull boxingWebNov 21, 2016 · Acetylation of small molecules is widespread in nature, and in some cases, cells use this process to detoxify harmful chemicals. Streptomyces species utilize a Gcn5 … small baking tins with aluminum lidsWebDec 5, 2024 · Phosphinothricin acetyltransferase ( pat) gene confers resistance to glufosinate by transforming this herbicide into N-acetyl-l-glufosinate (NAG). small baking dish sizes solihull breast clinicWebGmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) as selection marker for the transgenic line. small baking sheetsWebYour search returned 12 phosphinothricin acetyltransferase ELISA Kit across 1 supplier. Showing 1 of 1 suppliers (12 products total) > >> Select All. Select up to 5 products from … small balance form 5WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … solihull brickwork