Phosphinothricin acetyltransferase gene

Author: vvpi

August undefined, 2024

Webphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

Utility of the pat gene as a selectable marker gene in production of …

WebThe chimeric hygromycin phosphotransferase gene ( hph) and phosphinothricin acetyltransferase gene ( bar) have been used as selectable markers. The integration and expression of an hph gene in … WebApr 11, 2024 · DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. ... using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate … rawling road gateshead

Transgenic plants containing the phosphinothricin-N …

Web10.1007/BF00201636 Abstract L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via … Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from … rawling road bensham

2.3.1.183: phosphinothricin acetyltransferase - BRENDA

Phosphinothricin Acetyltransferase - an overview

WebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. WebCloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli Gene 63 65-74 1988 Streptomyces viridochromogenes, Streptomyces viridochromogenes Tue494 3290054. brenda. 673843. rawling home siteWebFeb 1, 2014 · The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [ 3, 56, 59, 63 ], have been used as selection markers in plant biology and engineering [ 11 ]. simple freight logistics

"WebMar 18, 2016 · Gene: BASTA selectable marker from Streptomyces hygrosopicus- phosphinothricin acetyltransferase gene to confer BASTA resistance as a selectable marker Promoter: 35S promoter from Cauliflower mosaic caulimovirus – CaMV 35S constitutive promoter to drive expression of GUS reporter gene " - Phosphinothricin acetyltransferase gene

Phosphinothricin acetyltransferase gene

Transgenic plants containing the phosphinothricin-N …

WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar WebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes.

Did you know?

WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase ( PAT) and bialaphos resistance ( bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.

WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said … WebThe gene encodes phosphinothricin acetyltransferase, which N -acetylates phosphinothricin using acetyl-CoA. Sign in to download full-size image Figure 6.1. Overview of biosynthetic pathways of a number of phosphonate and phosphinate natural products. The first two steps of phosphonate biosynthesis (boxed) are conserved for most known …

WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebFeb 16, 2016 · Wohlleben, W. et al. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70 , 25–37 (1988).

WebSKU: GENE-BT-CP4 Category: Foody Safety Assays Tag: plantExtract. Related products. Ovine DNA Real Time PCR Detection Kit. Read more. cp4Epsps Gene Rapid Test Kit. ... phosphinothricin-N-acetyltransferase Test pAT/cp4 Epsps combo Test. Scroll to top. This site uses cookies. By continuing to browse the site, you are agreeing to our use of ...

WebApr 11, 2024 · the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also … simple freight llcWebmechanism of Agrobacterium-mediated gene transfer has been widely utilized to genetically modify mono-cots and dicots (Hansen and Wright, 1999; Fukuoka et al., 2000). Agrobacterium tumefaciensis a soil-borne pathogen that has the capacity to infect plants and transfer a segment of DNA (T-DNA) from its tumor-inducing (Ti) plasmid to the plant … simple freight solutionsWebJun 15, 1992 · Cloning of a phosphinothricin N- acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli Gene (1988) P.G. Reddy et al. Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinobacter calcoaceticus RAG-1 Gene … simple freight solutions incWebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector … simple freezer jam without pectinWebIn this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. ... GmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) ... simple freezer meals for crockpotWebWohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TÜ494 and its expression in Nicotiana tabacum. Gene … rawling road pharmacyWebNational Center for Biotechnology Information simple freight solutions bohemia ny