Web1 aug. 2005 · Initiation of DNA replication from the Escherichia coli chromosomal origin is highly regulated, assuring that replication occurs precisely once per cell cycle. Three mechanisms for regulation of replication initiation have been proposed: titration of free DnaA initiator protein by the datA locus, sequestration of newly replicated origins by SeqA … Web12 The genome of E. coli consists of a single chromosome of approximately 4,6mbp. Initiation occurs in the origin of replication, oriC and proceeds bidirectionally until …
Hyperinitiation of DNA replication in Escherichia coli leads to ...
WebDNA microarray analyses showed that the level of initiation in rapidly growing cells that lack datA was indistinguishable from that in wild‐type cells, and that the absence of SeqA protein caused only a modest increase in initiation, in agreement with flow‐cytometry data. Initiation of DNA replication from the Escherichia coli chromosomal origin is highly regulated, … WebThe amount of collapsed forks is substantially higher under elevated expression of dnaAcos compared to that of dnaA. We propose that the lethal phenotype of chromosomally encoded dnaAcos is a result of hyperinitiation that overwhelms the repair capacity of the cell. comiskey group venues
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WebHyperinitiation of DNA replication in Escherichia coli leads to replication fork collapse and inviability Lyle A. Simmons , Department of Biochemistry and Molecular Biology, … Web1 mrt. 2010 · Interestingly, in a screen to isolate DnaA inhibitors a compound was recently found to rescue a dnaAcos mutant from lethal hyperinitiation by targeting gyrase [78]. Web1 aug. 2005 · Hyperinitiation of DNA replication in Escherichia coli leads to replication fork collapse and inviability Simmons, LA; Breier, AM; Cozzarelli, NR; Kaguni, JM Timing of … comiskey insurance galveston