WebTo simultaneously detect multiple target proteins in the In-Cell Western Assay, you must use secondary antibodies labeled with different fluorophores (e.g., IRDye 680RD Secondary Antibodies for 700 nm channel and IRDye 800CW Secondary Antibodies for 800 nm channel). Combined Detection of Pan Protein and Phospho-protein Levels WebJan 10, 2024 · Permeabilize with 0.1 % TX-100/PBS for 15–20 minutes and wash 3 ×. Block with 5 % normal goat serum/PBS or 1 % BSA/PBS for 45 minutes (no washing required). Dilute the primary antibody in blocking solution and apply it for 2 h (or overnight at 4 °C). Wash 4 × thoroughly to remove unbound primary antibody.
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WebGenetically encoded sensors provide an alternative to organic dyes for measuring redox potential or oxidative stress in live cells. roGFP is a fluorescent protein chimera that changes its excitation maximum from 488 nm to 400 nm based on … WebThe GE eXplore Optix scanner used in this study is a unique time-domain fluorescence imager for small rodents such as mice and rats. The instrument allows an operator to … small birds of south carolina
Whole-brain tissue mapping toolkit using large-scale highly ... - Nature
WebCeligo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. Celigo images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state. Web2. 'Image Export' will save the image in a format that other graphic software can open. A. In 'Input' window, choose the image to be exported. B. In 'Parameters' window, you can choose a presetting 'Export Fluorescent Image to 'D Drive' for fluorescent images, or 'Export Color Image to 'D Drive' for images taken with the color camera. WebDec 16, 2024 · This protocol (Figure 1) below describes the specific steps for labeling, imaging, and quantifying the number and somatic volume of neurons in intact mouse spinal cord segments (3–6mm). Use of the Labkit pixel classifier with Imaris visualization tools allows segmentation of cells in 3D even when fluorescent labeling is non-uniform as is ... solomon the magnificent